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Fiber-based Single-Pulse Fluorescence Lifetime Imaging Microscopy (SP-FLIM)

1 Jun | By Sebastian Karpf
Fiber-based Single-Pulse Fluorescence Lifetime Imaging Microscopy (SP-FLIM)
2P-FLIM image of convallaria majalis plant stem
Image source: https://www.osapublishing.org/BOE/abstract.cfm?uri=BOE-8-7-3132
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Researchers from University of Lübeck in Germany and University of California, Los Angeles (UCLA) in the USA have presented a system for fiber-based Two-Photon Fluorescence Lifetime Imaging Microscopy (2P-FLIM). Their findings are featured as an editor's pick in the upcoming issue of Biomedical Optics Express. According to the journal's statement, "Editor's Picks serve to highlight articles with excellent scientific quality and are representative of the work taking place in a specific field".

In their manuscript "Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate"the researchers around Professor Robert Huber report on a new system for rapid Two-Photon Lifetime Imaging using only a single excitation pulse per pixel. In comparison to the current gold standard, Time-Correlated Single-Photon Counting (TCSPC), this approach can reach orders of magnitude faster imaging speeds. A main advantage is the fiber-based laser source which is rendered possible by the relatively longer picosecond to nanosecond-regime laser pulses used. Such a fiber-based system may readily be employed to endoscopic applications of 2P-FLIM for tumour margin detection, cellular metabolic monitoring or circulating tumour cell (CTC) detection during metastases stage.

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