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Intravital multiphoton photoconversion with a cell membrane dye

26 Apr | By Biophotonics.World
Intravital multiphoton photoconversion with a cell membrane dye
Image source: Journal of Biophotonics
Photoconversion, an irreversible shift in a fluorophore emission spectrum after light exposure, is a powerful tool for marking cellular and subcellular compartments and tracking their dynamics in vivo. This paper reports on the photoconversion properties of Di-8-ANEPPS, a commercially available membrane dye. When illuminated with near-infrared femtosecond laser pulses, Di-8-ANEPPS undergoes multiphoton photoconversion as indicated by the supralinear dependence of the conversion rate on the incident power, and by the ability to photoconvert a thin optical section in a three-dimensional matrix. The characteristic emission spectrum changed from red to blue, and ratiometric analysis on single cells in vitro revealed a 65-fold increase in the blue to red wavelength ratio after photoconversion. The spectral shift is preserved in vivo for hours, making Di-8-ANEPPS a useful dye for intravital cell marking and tracking applications. The article was published by Raphaël Turcotte et al. in J. Biophotonics, 2017, 2, 206; DOI: 10.1002/jbio.201600077 
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